%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Sean J. Blamires, Madeleine Nobbs, Penny J. Martens, I-Min Tso, Wei-Tsung Chuang, Chung-Kai Chang, Hwo-Shuenn Sheu
Multiscale mechanisms of nutritionally induced property variation in spider silks
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 12 0 R/Annots 13 0 R/Contents 14 0 R/TrimBox[0 0 612 792]>>
endobj
13 0 obj
[15 0 R 16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R]
endobj
15 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref005)>>
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref005)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref005)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref006)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref007)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref011)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref011)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref015)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref011)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref014)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref011)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref016)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref017)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref018)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref018)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref019)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref020)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref021)>>
endobj
33 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref022)>>
endobj
34 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref023)>>
endobj
35 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref011)>>
endobj
36 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref010)>>
endobj
37 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref010)>>
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref016)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref024)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref025)>>
endobj
41 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
42 0 obj
<>/Border[0 0 0]/A 43 0 R>>
endobj
43 0 obj
<>
endobj
14 0 obj
[44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R 62 0 R]
endobj
44 0 obj
<>stream
q
0.83 0.64 0.02 0 k
475.1433 693.2976 m
479.9055 693.2976 l
h
f*
456.2079 641.3102 m
460.9701 641.3102 l
h
f*
260.1638 615.3449 m
264.926 615.3449 l
h
f*
267.1937 615.3449 m
271.9559 615.3449 l
h
f*
345.6 602.3055 m
350.3622 602.3055 l
h
f*
355.5213 602.3055 m
365.0457 602.3055 l
h
f*
386.8724 589.3228 m
396.3969 589.3228 l
h
f*
401.5559 589.3228 m
411.0803 589.3228 l
h
f*
203.4142 537.3354 m
212.9386 537.3354 l
h
f*
215.1496 537.3354 m
224.674 537.3354 l
h
f*
407.0551 472.3087 m
416.5795 472.3087 l
h
f*
418.8472 472.3087 m
428.3717 472.3087 l
h
f*
203.4142 446.3433 m
212.9386 446.3433 l
h
f*
215.1496 446.3433 m
224.674 446.3433 l
h
f*
203.4142 407.3386 m
212.9386 407.3386 l
h
f*
215.1496 407.3386 m
224.674 407.3386 l
h
f*
357.9591 381.3165 m
367.4835 381.3165 l
h
f*
525.0898 368.3339 m
534.6142 368.3339 l
h
f*
482.1732 342.3118 m
491.6976 342.3118 l
h
f*
493.9654 342.3118 m
503.4898 342.3118 l
h
f*
542.2677 290.3244 m
551.7921 290.3244 l
h
f*
460.063 277.3417 m
469.5307 277.3417 l
h
f*
403.2 199.3323 m
412.7244 199.3323 l
h
f*
414.9921 199.3323 m
424.4598 199.3323 l
h
f*
426.7276 199.3323 m
436.252 199.3323 l
h
f*
319.9181 160.3276 m
329.4425 160.3276 l
h
f*
334.6016 160.3276 m
344.126 160.3276 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(such)Tj
2.0579 0 Td
(fibers)Tj
2.4378 0 Td
(is)Tj
0.8504 0 Td
(that)Tj
1.7744 0 Td
(the)Tj
1.474 0 Td
(properties)Tj
4.2747 0 Td
(of)Tj
1.0148 0 Td
(MA)Tj
1.7971 0 Td
(silk)Tj
1.5931 0 Td
(are)Tj
1.4456 0 Td
(highly)Tj
2.7099 0 Td
(variable)Tj
3.3562 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.474 0 Td
(mechanisms)Tj
5.2043 0 Td
(inducing)Tj
-33.1934 -1.3039 Td
(this)Tj
1.6724 0 Td
(variation)Tj
3.8041 0 Td
(have)Tj
2.0579 0 Td
(never)Tj
2.4434 0 Td
(been)Tj
2.1203 0 Td
(delineated)Tj
4.3427 0 Td
(from)Tj
2.2053 0 Td
(nano)Tj
2.2564 0 Td
(to)Tj
1.0261 0 Td
(macro)Tj
2.7723 0 Td
(scales)Tj
2.4718 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
-25.9765 -1.2982 Td
(Researchers)Tj
4.9662 0 Td
(can)Tj
1.6214 0 Td
(determine)Tj
4.3257 0 Td
(the)Tj
1.4683 0 Td
(consequences)Tj
5.6976 0 Td
(of)Tj
1.0148 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4674 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(functional)Tj
4.3087 0 Td
(proper-)Tj
-33.8908 -1.2983 Td
(ties)Tj
1.5703 0 Td
(of)Tj
1.0148 0 Td
(structural)Tj
4.0762 0 Td
(proteins)Tj
3.515 0 Td
(by)Tj
1.1848 0 Td
(either)Tj
2.5285 0 Td
(switching)Tj
4.0649 0 Td
(genes)Tj
2.4378 0 Td
(on)Tj
1.2755 0 Td
(and)Tj
1.7348 0 Td
(off)Tj
1.2983 0 Td
(and)Tj
1.7348 0 Td
(observing)Tj
4.1215 0 Td
(the)Tj
1.4684 0 Td
(consequences)Tj
-32.0256 -1.2983 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(secreted)Tj
3.4695 0 Td
(proteins,)Tj
3.7418 0 Td
(or)Tj
1.0998 0 Td
(by)Tj
1.1792 0 Td
(observing)Tj
4.1272 0 Td
(the)Tj
1.4684 0 Td
(function)Tj
3.6283 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(proteins)Tj
3.5149 0 Td
(produced)Tj
4.0479 0 Td
(in)Tj
1.0318 0 Td
(different)Tj
-32.2977 -1.3039 Td
(organisms)Tj
4.3539 0 Td
(with)Tj
1.9956 0 Td
(varying)Tj
3.2145 0 Td
(levels)Tj
2.3754 0 Td
(of)Tj
1.0148 0 Td
(expression)Tj
4.4674 0 Td
(of)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(particular)Tj
4.1102 0 Td
(gene)Tj
2.0807 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
1.6044 0 Td
(Indeed,)Tj
3.2371 0 Td
(using)Tj
2.3868 0 Td
(such)Tj
-32.5075 -1.2982 Td
(approaches)Tj
4.7338 0 Td
(has)Tj
1.542 0 Td
(informed)Tj
3.9742 0 Td
(us)Tj
1.1111 0 Td
(how)Tj
1.9389 0 Td
(and)Tj
1.7291 0 Td
(why)Tj
1.8879 0 Td
(certain)Tj
2.982 0 Td
(genes)Tj
2.4435 0 Td
(cause)Tj
2.3924 0 Td
(specific)Tj
3.1861 0 Td
(ailments)Tj
3.6227 0 Td
(in)Tj
1.0318 0 Td
(humans)Tj
3.4412 0 Td
(and)Tj
-36.0167 -1.2983 Td
(other)Tj
2.347 0 Td
(animals)Tj
3.3279 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(].)Tj
2.3017 0 Td
(Progress)Tj
3.6397 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(recently)Tj
3.4072 0 Td
(made)Tj
2.4264 0 Td
(into)Tj
1.8425 0 Td
(our)Tj
1.6271 0 Td
(understanding)Tj
6.0661 0 Td
(of)Tj
1.0148 0 Td
(spider)Tj
2.6872 0 Td
(silk)Tj
-34.3556 -1.3039 Td
(genes)Tj
2.4377 0 Td
(and)Tj
1.7348 0 Td
(their)Tj
2.109 0 Td
(expression)Tj
4.4617 0 Td
(patterns)Tj
3.4752 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
3.0671 0 Td
(Likewise,)Tj
3.9061 0 Td
(significant)Tj
4.388 0 Td
(insights)Tj
3.3165 0 Td
(have)Tj
2.058 0 Td
(been)Tj
2.126 0 Td
(gained)Tj
2.8856 0 Td
(on)Tj
-35.9657 -1.2983 Td
(silk)Tj
1.5873 0 Td
(production,)Tj
4.9436 0 Td
(spinning,)Tj
3.9685 0 Td
(and)Tj
1.7291 0 Td
(its)Tj
1.1452 0 Td
(engineering)Tj
4.972 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
3.5489 0 Td
(However,)Tj
4.0762 0 Td
(because)Tj
3.3222 0 Td
(no)Tj
1.2756 0 Td
(single)Tj
2.5285 0 Td
(study)Tj
2.3924 0 Td
(has)Tj
-35.4895 -1.2983 Td
(holistically)Tj
4.4957 0 Td
(examined)Tj
4.1329 0 Td
(the)Tj
1.4683 0 Td
(consequences)Tj
5.6976 0 Td
(of)Tj
1.0148 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4674 0 Td
(on)Tj
1.2756 0 Td
(protein)Tj
3.1521 0 Td
(structure)Tj
3.8211 0 Td
(and)Tj
1.7291 0 Td
(silk)Tj
1.593 0 Td
(func-)Tj
-34.9282 -1.3039 Td
(tional)Tj
2.5171 0 Td
(properties,)Tj
4.5071 0 Td
(it)Tj
0.7823 0 Td
(is)Tj
0.8504 0 Td
(not)Tj
1.5761 0 Td
(known)Tj
3.0047 0 Td
(why)Tj
1.8822 0 Td
(spiders)Tj
3.05 0 Td
(spin)Tj
1.9162 0 Td
(silks)Tj
1.9502 0 Td
(with)Tj
1.9956 0 Td
(such)Tj
2.0636 0 Td
(exceptional)Tj
4.7735 0 Td
(properties)Tj
4.2747 0 Td
(and,)Tj
-35.1437 -1.2982 Td
(more)Tj
2.3414 0 Td
(importantly,)Tj
5.2043 0 Td
(why)Tj
1.8879 0 Td
(silk)Tj
1.5931 0 Td
(properties)Tj
4.2746 0 Td
(vary)Tj
1.9389 0 Td
(so)Tj
1.0828 0 Td
(much)Tj
2.5228 0 Td
(between)Tj
3.5263 0 Td
(and)Tj
1.7291 0 Td
(within)Tj
2.8176 0 Td
(individual)Tj
4.286 0 Td
(spiders)Tj
-33.2048 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
3.2541 0 Td
(Experimentally)Tj
6.3042 0 Td
(switching)Tj
4.0592 0 Td
(silk)Tj
1.5931 0 Td
(related)Tj
2.9423 0 Td
(genes)Tj
2.4435 0 Td
(on)Tj
1.2755 0 Td
(and)Tj
1.7292 0 Td
(off)Tj
1.3039 0 Td
(within)Tj
2.8119 0 Td
(individual)Tj
4.2917 0 Td
(spiders)Tj
3.0444 0 Td
(is)Tj
0.8503 0 Td
(not)Tj
-35.9033 -1.3039 Td
(yet)Tj
1.3946 0 Td
(achievable,)Tj
4.5864 0 Td
(so)Tj
1.0885 0 Td
(observing)Tj
4.1272 0 Td
(the)Tj
1.4684 0 Td
(function)Tj
3.6283 0 Td
(of)Tj
1.0148 0 Td
(silk)Tj
1.593 0 Td
(proteins)Tj
3.5093 0 Td
(in)Tj
1.0375 0 Td
(different)Tj
3.6567 0 Td
(spiders)Tj
3.05 0 Td
(with)Tj
1.9956 0 Td
(varying)Tj
3.2145 0 Td
(levels)Tj
-35.3648 -1.2983 Td
(of)Tj
1.0147 0 Td
(expression)Tj
4.4618 0 Td
(of)Tj
1.0147 0 Td
(particular)Tj
4.1159 0 Td
(genes)Tj
2.4378 0 Td
(appears)Tj
3.2768 0 Td
(the)Tj
1.474 0 Td
(best)Tj
1.8085 0 Td
(way)Tj
1.7915 0 Td
(forward.)Tj
-20.1995 -1.2982 Td
(MA)Tj
1.7971 0 Td
(silk)Tj
1.5931 0 Td
(properties)Tj
4.2803 0 Td
(have)Tj
2.0579 0 Td
(traditionally)Tj
5.1193 0 Td
(been)Tj
2.1203 0 Td
(thought)Tj
3.3733 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1508 0 Td
(the)Tj
1.4684 0 Td
(product)Tj
3.3959 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(combined)Tj
-31.0618 -1.2983 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(genes)Tj
2.4434 0 Td
(coding)Tj
2.9594 0 Td
(for)Tj
1.3889 0 Td
(two)Tj
1.7065 0 Td
(proteins)Tj
3.5149 0 Td
(\(called)Tj
2.863 0 Td
(spidroins\);)Tj
4.5127 0 Td
(major)Tj
2.6079 0 Td
(ampullate)Tj
4.1669 0 Td
(spidroin)Tj
3.5886 0 Td
(1,)Tj
0.9184 0 Td
(or)Tj
-36.1471 -1.3039 Td
(MaSp1,)Tj
3.2371 0 Td
(and)Tj
1.7348 0 Td
(major)Tj
2.6078 0 Td
(ampullate)Tj
4.1669 0 Td
(spidroin)Tj
3.583 0 Td
(2,)Tj
0.9241 0 Td
(or)Tj
1.0998 0 Td
(MaSp2)Tj
3.0104 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(])Tj
3.0331 0 Td
(\(with)Tj
2.3357 0 Td
(the)Tj
1.4683 0 Td
(genes)Tj
2.4435 0 Td
(called)Tj
/F11 1 Tf
2.5171 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9764 0 Td
(and)Tj
/F11 1 Tf
-35.138 -1.2983 Td
[()28()14()]TJ
/F6 1 Tf
(\).)Tj
3.5376 0 Td
(The)Tj
1.7801 0 Td
(secondary)Tj
4.2746 0 Td
(structures)Tj
4.1839 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(spidroins)Tj
3.9458 0 Td
(are)Tj
1.4513 0 Td
(considered)Tj
4.5978 0 Td
(critical)Tj
2.9537 0 Td
(for)Tj
1.3889 0 Td
(silk)Tj
1.5874 0 Td
(performance)Tj
-32.1843 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
3.2541 0 Td
(MaSp1)Tj
3.0104 0 Td
(consists)Tj
3.3505 0 Td
(of)Tj
1.0148 0 Td
(repeating)Tj
3.9685 0 Td
(polyalanine,)Tj
5.074 0 Td
(\(GA\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 417.5433 445.7196 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
[()]TJ
/F6 1 Tf
(,)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 425.3669 447.4204 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(GGX\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 452.9763 445.7196 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
[()]TJ
ET
Q
q
1 j
1 J
0 w
10 0 0 10 459.1559 447.4204 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(\(A\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 490.0535 445.7196 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
[()]TJ
ET
Q
q
1 j
1 J
0 w
10 0 0 10 496.233 447.4204 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(amino)Tj
2.7949 0 Td
(acid)Tj
1.8708 0 Td
(motifs)Tj
-34.2875 -1.3039 Td
(\(G)Tj
1.2756 0 Td
(=)Tj
0.7993 0 Td
(glycine,)Tj
3.2712 0 Td
(A)Tj
0.9071 0 Td
(=)Tj
0.7993 0 Td
(alanine)Tj
3.1238 0 Td
(and)Tj
1.7291 0 Td
(X)Tj
0.8731 0 Td
(=)Tj
0.7994 0 Td
(other)Tj
2.347 0 Td
(amino)Tj
2.795 0 Td
(acids\).)Tj
2.8006 0 Td
(These)Tj
2.5682 0 Td
(motifs)Tj
2.7609 0 Td
(form)Tj
2.2053 0 Td
(cassettes)Tj
3.5999 0 Td
(that)Tj
1.7801 0 Td
(com-)Tj
-34.4349 -1.2982 Td
(bine)Tj
1.9672 0 Td
(to)Tj
1.0205 0 Td
(promote)Tj
3.668 0 Td
(the)Tj
1.4683 0 Td
(formation)Tj
4.2576 0 Td
(of)Tj
1.0148 0 Td
(crystalline)Tj
/F12 1 Tf
4.2973 0 Td
()Tj
/F6 1 Tf
(sheet)Tj
3.2599 0 Td
(nanostructures)Tj
6.2191 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4682 0 Td
(assembled)Tj
4.3257 0 Td
(fibers)Tj
-34.0041 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
3.2541 0 Td
(The)Tj
1.7802 0 Td
(MaSp2)Tj
3.016 0 Td
(protein)Tj
3.1521 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(other)Tj
2.347 0 Td
(hand)Tj
2.2621 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.1203 0 Td
(thought)Tj
3.3732 0 Td
(to)Tj
1.0261 0 Td
(consist)Tj
2.9877 0 Td
(of)Tj
1.0147 0 Td
(multiple)Tj
-30.6195 -1.3039 Td
(\(GPGXX\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 239.7543 393.7322 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
[()]TJ
ET
Q
q
1 j
1 J
0 w
10 0 0 10 245.9338 395.3763 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(motifs)Tj
2.7609 0 Td
(\(where)Tj
2.9877 0 Td
(P)Tj
0.7767 0 Td
(=)Tj
0.7993 0 Td
(proline\),)Tj
3.668 0 Td
(and)Tj
1.7292 0 Td
(predicted)Tj
4.0025 0 Td
(to)Tj
1.0261 0 Td
(form)Tj
2.2053 0 Td
(disordered)Tj
4.5298 0 Td
(type)Tj
1.9105 0 Td
(II)Tj
/F12 1 Tf
0.9014 0 Td
()Tj
/F6 1 Tf
(-turns)Tj
3.1635 0 Td
(and)Tj
-35.0529 -1.2982 Td
(similar)Tj
2.9877 0 Td
(non-crystalline)Tj
6.2474 0 Td
(nanostructures)Tj
6.2191 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.0807 0 Td
(Collectively,)Tj
5.0966 0 Td
(the)Tj
1.4684 0 Td
(various)Tj
3.1464 0 Td
(nanostructures)Tj
6.2192 0 Td
(are)Tj
-33.4655 -1.2983 Td
(thought)Tj
3.3732 0 Td
(to)Tj
1.0204 0 Td
(combine)Tj
3.719 0 Td
(and)Tj
1.7291 0 Td
(provide)Tj
3.2881 0 Td
(MA)Tj
1.7972 0 Td
(silk)Tj
1.5931 0 Td
(with)Tj
1.9955 0 Td
(its)Tj
1.1452 0 Td
(great)Tj
2.211 0 Td
(strength)Tj
3.515 0 Td
(and)Tj
1.7348 0 Td
(extensibility)Tj
5.0456 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.075 0 Td
(Since)Tj
-34.2422 -1.2982 Td
(MaSp2)Tj
3.016 0 Td
(has)Tj
1.542 0 Td
(long)Tj
1.9842 0 Td
(been)Tj
2.1259 0 Td
(predicted)Tj
3.9969 0 Td
(to)Tj
1.0261 0 Td
(contain)Tj
3.2485 0 Td
(\(GPGXX\))Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 409.1527 354.7275 cm
BT
/F11 1 Tf
1 TL
-0.0053 Tc
0 0 Td
[()]TJ
ET
Q
q
1 j
1 J
0 w
10 0 0 10 415.2755 356.4283 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(sequences,)Tj
4.4446 0 Td
(the)Tj
1.4741 0 Td
(proline)Tj
3.101 0 Td
(composition)Tj
5.2384 0 Td
(of)Tj
-35.7842 -1.3039 Td
(MA)Tj
1.7971 0 Td
(silk)Tj
1.5874 0 Td
(was)Tj
1.7008 0 Td
(considered)Tj
4.6034 0 Td
(a)Tj
0.652 0 Td
(reliable)Tj
3.1408 0 Td
(indicator)Tj
3.8607 0 Td
(of)Tj
/F11 1 Tf
1.0148 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9764 0 Td
(gene)Tj
2.0805 0 Td
(expression)Tj
4.4617 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
3.2542 0 Td
(The)Tj
1.7858 0 Td
(ratio)Tj
2.0976 0 Td
(of)Tj
/F11 1 Tf
-35.0132 -1.2982 Td
[()28()14()]TJ
/F6 1 Tf
(:)Tj
/F11 1 Tf
3.1974 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9764 0 Td
(expression)Tj
4.4617 0 Td
(is)Tj
0.8504 0 Td
(variable)Tj
3.3562 0 Td
(among)Tj
2.9934 0 Td
(and)Tj
1.7291 0 Td
(between)Tj
3.5318 0 Td
(spider)Tj
2.6873 0 Td
(species,)Tj
3.2201 0 Td
(presumably)Tj
4.8812 0 Td
(because)Tj
-33.885 -1.2983 Td
(the)Tj
1.4683 0 Td
(MaSp2)Tj
3.0161 0 Td
(protein)Tj
3.1521 0 Td
(is)Tj
0.8504 0 Td
(metabolically)Tj
5.5275 0 Td
(costly)Tj
2.5058 0 Td
(to)Tj
1.0261 0 Td
(synthesize)Tj
4.2972 0 Td
(so)Tj
1.0885 0 Td
(may)Tj
1.9276 0 Td
(be)Tj
1.1508 0 Td
(differentially)Tj
5.3065 0 Td
(expressed.)Tj
4.3199 0 Td
(Var-)Tj
-35.6368 -1.3039 Td
(iation)Tj
2.5398 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(ratio)Tj
2.0976 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(two)Tj
1.7121 0 Td
(spidroins)Tj
3.9458 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(traditionally)Tj
5.1192 0 Td
(thought)Tj
3.3733 0 Td
(to)Tj
1.0261 0 Td
(bring)Tj
2.3754 0 Td
(about)Tj
2.4945 0 Td
(variations)Tj
-33.3408 -1.2983 Td
(in)Tj
1.0318 0 Td
(nanostructure)Tj
5.862 0 Td
(formations)Tj
4.6204 0 Td
(leading)Tj
3.1351 0 Td
(to)Tj
1.0262 0 Td
(variations)Tj
4.1612 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4682 0 Td
(mechanics)Tj
4.4504 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(spun)Tj
2.177 0 Td
(fibres)Tj
2.4378 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(].)Tj
-32.6945 -1.2982 Td
(The)Tj
1.7858 0 Td
(recent)Tj
2.7099 0 Td
(mapping)Tj
3.7871 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F11 1 Tf
1.4683 0 Td
[()28( )2()10(\n)18()4()4()]TJ
3.2768 0 Td
[(\r)13()4()22()-13()10()4( )8()]TJ
/F6 1 Tf
(')Tj
3.4299 0 Td
(spidroin)Tj
3.5886 0 Td
(genome)Tj
3.4072 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(])Tj
1.8482 0 Td
(has)Tj
1.542 0 Td
(caused)Tj
2.9197 0 Td
(researchers)Tj
4.7111 0 Td
(to)Tj
-36.6856 -1.304 Td
(rethink)Tj
3.1521 0 Td
(much)Tj
2.5171 0 Td
(assumed)Tj
3.6737 0 Td
(knowledge.)Tj
4.7679 0 Td
(We)Tj
1.61 0 Td
(now)Tj
1.9616 0 Td
(understand)Tj
4.7847 0 Td
(that,)Tj
2.007 0 Td
(at)Tj
0.9524 0 Td
(least)Tj
1.9842 0 Td
(in)Tj
/F11 1 Tf
1.0318 0 Td
[()]TJ
/F6 1 Tf
(.)Tj
/F11 1 Tf
1.1396 0 Td
[(\r)13()4()22()-13()10()4( )8()]TJ
/F6 1 Tf
(:)Tj
3.4412 0 Td
(\(i\))Tj
1.1679 0 Td
(individ-)Tj
-34.1912 -1.2982 Td
(ual)Tj
1.4286 0 Td
(spiders)Tj
3.0444 0 Td
(express)Tj
3.1465 0 Td
(multiple)Tj
3.5659 0 Td
(versions)Tj
3.515 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F11 1 Tf
1.4683 0 Td
[()28()14()]TJ
/F6 1 Tf
2.5058 0 Td
(genes)Tj
2.4434 0 Td
(at)Tj
0.9524 0 Td
(different)Tj
3.6567 0 Td
(loci,)Tj
1.8878 0 Td
(potentially)Tj
4.4617 0 Td
(leading)Tj
3.1351 0 Td
(to)Tj
-36.2264 -1.2983 Td
(several)Tj
2.931 0 Td
(MaSp1)Tj
3.016 0 Td
(and)Tj
1.7292 0 Td
(MaSp2)Tj
3.016 0 Td
(proteins)Tj
3.5149 0 Td
(appearing)Tj
4.201 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(spun)Tj
2.1712 0 Td
(silks,)Tj
2.177 0 Td
(\(ii\))Tj
1.4343 0 Td
(the)Tj
1.4683 0 Td
(different)Tj
3.6567 0 Td
(spidroin)Tj
3.5887 0 Td
(cas-)Tj
-35.4101 -1.3039 Td
(settes)Tj
2.3867 0 Td
(and)Tj
1.7292 0 Td
(motifs)Tj
2.7609 0 Td
(are)Tj
1.4457 0 Td
(shared)Tj
2.8686 0 Td
(and)Tj
1.7291 0 Td
(partitioned)Tj
4.6772 0 Td
(among)Tj
2.999 0 Td
(the)Tj
1.4682 0 Td
(different)Tj
3.6567 0 Td
(proteins,)Tj
3.736 0 Td
(and)Tj
1.7348 0 Td
(\(iii\))Tj
1.7008 0 Td
(other)Tj
2.3471 0 Td
(spi-)Tj
-35.24 -1.2983 Td
(droins,)Tj
3.0274 0 Td
(e.g.)Tj
1.559 0 Td
(AcSps,)Tj
2.9083 0 Td
(appear)Tj
2.914 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(major)Tj
2.6078 0 Td
(ampullate)Tj
4.1669 0 Td
(gland,)Tj
2.6701 0 Td
(so)Tj
1.0885 0 Td
(can)Tj
1.6214 0 Td
(provide)Tj
3.2939 0 Td
(additional)Tj
4.2746 0 Td
(cassettes)Tj
-32.6378 -1.2982 Td
(and)Tj
1.7291 0 Td
(motifs)Tj
2.7609 0 Td
(that)Tj
1.7802 0 Td
(may)Tj
1.9275 0 Td
(form)Tj
2.2054 0 Td
(components)Tj
5.176 0 Td
(of)Tj
1.0148 0 Td
(MA)Tj
1.7972 0 Td
(silk)Tj
1.5873 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
4.4333 0 Td
(For)Tj
1.6214 0 Td
(these)Tj
2.2564 0 Td
(reasons,)Tj
3.4525 0 Td
(MA)Tj
1.7972 0 Td
(silk)Tj
-33.5392 -1.2983 Td
(amino)Tj
2.7949 0 Td
(acid)Tj
1.8709 0 Td
(compositions)Tj
5.6069 0 Td
(may)Tj
1.9275 0 Td
(not)Tj
1.5761 0 Td
(necessarily)Tj
4.5297 0 Td
(reflect)Tj
2.6986 0 Td
(the)Tj
/F11 1 Tf
1.4682 0 Td
[()28()14()]TJ
/F6 1 Tf
(:)Tj
/F11 1 Tf
3.1975 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9763 0 Td
(expression,)Tj
4.6885 0 Td
(possibly)Tj
-33.3351 -1.3039 Td
(explaining)Tj
4.3993 0 Td
(why)Tj
1.8879 0 Td
(nanostructure)Tj
5.862 0 Td
(formations)Tj
4.6204 0 Td
(can)Tj
1.6271 0 Td
(vary)Tj
1.9332 0 Td
(within)Tj
2.8119 0 Td
(individual)Tj
4.2916 0 Td
(spider)Tj
2.6872 0 Td
(silks)Tj
1.9503 0 Td
(independent)Tj
-32.0709 -1.2983 Td
(of)Tj
/F11 1 Tf
1.0148 0 Td
[()28()14()]TJ
/F6 1 Tf
(:)Tj
/F11 1 Tf
3.1974 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9764 0 Td
(expression)Tj
4.4617 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
-10.4541 -1.2982 Td
(Any)Tj
1.9105 0 Td
(study)Tj
2.3868 0 Td
(aspiring)Tj
3.4526 0 Td
(to)Tj
1.0261 0 Td
(understand)Tj
4.7905 0 Td
(how)Tj
1.9389 0 Td
(spidroin)Tj
3.583 0 Td
(gene)Tj
2.0805 0 Td
(expression)Tj
4.4674 0 Td
(influences)Tj
4.2803 0 Td
(MA)Tj
1.7971 0 Td
(silk)Tj
1.5874 0 Td
(nano-)Tj
-34.4973 -1.304 Td
(structure)Tj
3.8211 0 Td
(formation)Tj
4.2632 0 Td
(must)Tj
2.2224 0 Td
(reliably)Tj
3.1748 0 Td
(measure)Tj
3.5773 0 Td
(the)Tj
1.4683 0 Td
(size,)Tj
1.9106 0 Td
(density,)Tj
3.3277 0 Td
(orientation,)Tj
4.9153 0 Td
(and)Tj
1.7291 0 Td
(distances)Tj
3.8664 0 Td
(between)Tj
-34.2762 -1.2982 Td
(the)Tj
1.4683 0 Td
(crystalline)Tj
4.2973 0 Td
(and)Tj
1.7291 0 Td
(non-crystalline)Tj
6.2476 0 Td
(nanostructures)Tj
6.2191 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4682 0 Td
(silk)Tj
1.5931 0 Td
(proteins)Tj
3.5149 0 Td
(in)Tj
1.0375 0 Td
(addition)Tj
3.5943 0 Td
(to)Tj
1.0262 0 Td
(genetic)Tj
-33.2331 -1.2983 Td
(expression.)Tj
4.6885 0 Td
(Techniques)Tj
4.8415 0 Td
(such)Tj
2.0579 0 Td
(as)Tj
1.0148 0 Td
(small)Tj
2.3301 0 Td
(angle)Tj
2.3357 0 Td
(X-ray)Tj
2.4832 0 Td
(scattering)Tj
4.1101 0 Td
(\(SAXS\),)Tj
3.4129 0 Td
(wide)Tj
2.1259 0 Td
(angle)Tj
2.3358 0 Td
(X-ray)Tj
2.4831 0 Td
(scatter-)Tj
-34.2195 -1.3039 Td
(ing)Tj
1.5023 0 Td
(\(WAXS\),)Tj
3.9005 0 Td
(nuclear)Tj
3.1974 0 Td
(magnetic)Tj
3.9005 0 Td
(resonance)Tj
4.2576 0 Td
(\(NMR\),)Tj
3.3789 0 Td
(Fourier)Tj
3.2144 0 Td
(transform)Tj
4.2179 0 Td
(infrared)Tj
3.4526 0 Td
(\(FTIR\),)Tj
3.2258 0 Td
(Raman)Tj
-34.2479 -1.2983 Td
(and)Tj
1.7291 0 Td
(circular)Tj
3.2825 0 Td
(dichroism)Tj
4.2916 0 Td
(\(CD\))Tj
2.3018 0 Td
(spectroscopy,)Tj
5.6125 0 Td
(and)Tj
1.7291 0 Td
(transmission)Tj
5.3631 0 Td
(electron)Tj
3.4639 0 Td
(microscopy,)Tj
5.0966 0 Td
(have)Tj
2.058 0 Td
(been)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 388.5732 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Multiscale)Tj
4.585 0 Td
(mechanism)Tj
5.0316 0 Td
(s)Tj
0.7157 0 Td
(of)Tj
1.0418 0 Td
(spider)Tj
2.8913 0 Td
(silk)Tj
1.63 0 Td
(property)Tj
3.8268 0 Td
(variatio)Tj
3.1535 0 Td
(n)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01920)Tj
4.089 0 Td
(05)Tj
0 g
2.0906 0 Td
(February)Tj
4.1599 0 Td
(1,)Tj
1.0417 0 Td
(2018)Tj
33.7042 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(23)Tj
/F8 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F9 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3748 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
45 0 obj
<>stream
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
endstream
endobj
54 0 obj
<>stream
endstream
endobj
55 0 obj
<>stream
endstream
endobj
56 0 obj
<>stream
endstream
endobj
57 0 obj
<>stream
endstream
endobj
58 0 obj
<>stream
endstream
endobj
59 0 obj
<>stream
endstream
endobj
60 0 obj
<>stream
endstream
endobj
61 0 obj
<>stream
endstream
endobj
62 0 obj
<>stream
BT
10.3394 82.7156 Td
(exist.)Tj
ET
Q
endstream
endobj
63 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 12 0 R/Annots 65 0 R/Contents 66 0 R/TrimBox[0 0 612 792]>>
endobj
65 0 obj
[67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R 73 0 R 74 0 R 75 0 R 76 0 R 77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R 87 0 R 88 0 R 89 0 R 90 0 R 91 0 R 92 0 R 93 0 R 94 0 R 95 0 R 96 0 R 97 0 R 98 0 R 99 0 R 100 0 R 101 0 R 102 0 R]
endobj
67 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref028)>>
endobj
68 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref031)>>
endobj
69 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref032)>>
endobj
70 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref033)>>
endobj
71 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref034)>>
endobj
72 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref036)>>
endobj
73 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref028)>>
endobj
74 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref037)>>
endobj
75 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref038)>>
endobj
76 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref037)>>
endobj
77 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref039)>>
endobj
78 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref041)>>
endobj
79 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref037)>>
endobj
80 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref041)>>
endobj
81 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref042)>>
endobj
82 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref041)>>
endobj
83 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref043)>>
endobj
84 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref043)>>
endobj
85 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref044)>>
endobj
86 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref030)>>
endobj
87 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref043)>>
endobj
88 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref025)>>
endobj
89 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref025)>>
endobj
90 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref045)>>
endobj
91 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref046)>>
endobj
92 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref047)>>
endobj
93 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref050)>>
endobj
94 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref046)>>
endobj
95 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
96 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref025)>>
endobj
97 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
98 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
99 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref025)>>
endobj
100 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
101 0 obj
<>/Border[0 0 0]/Dest(Rpone.0192005.ref027)>>
endobj
102 0 obj
<>/Border[0 0 0]/A 103 0 R>>
endobj
103 0 obj
<>
endobj
66 0 obj
[104 0 R 105 0 R 106 0 R 107 0 R 108 0 R 109 0 R 110 0 R 111 0 R 112 0 R 113 0 R 114 0 R 115 0 R 116 0 R 117 0 R 118 0 R 119 0 R 120 0 R 121 0 R 122 0 R 123 0 R 124 0 R]
endobj
104 0 obj
<>stream
q
0.83 0.64 0.02 0 k
432.1134 706.337 m
441.6378 706.337 l
h
f*
446.7969 706.337 m
456.3213 706.337 l
h
f*
328.1386 667.3323 m
337.6063 667.3323 l
h
f*
339.874 667.3323 m
349.3984 667.3323 l
h
f*
334.6583 641.3102 m
344.1827 641.3102 l
h
f*
349.3417 641.3102 m
358.8661 641.3102 l
h
f*
227.8488 602.3055 m
237.3732 602.3055 l
h
f*
239.5843 602.3055 m
249.1087 602.3055 l
h
f*
251.3764 602.3055 m
260.9008 602.3055 l
h
f*
345.7134 576.3402 m
355.2378 576.3402 l
h
f*
357.4488 576.3402 m
366.9732 576.3402 l
h
f*
372.1323 576.3402 m
381.6567 576.3402 l
h
f*
433.8142 550.3181 m
443.2819 550.3181 l
h
f*
445.5496 550.3181 m
455.074 550.3181 l
h
f*
457.3417 550.3181 m
466.8661 550.3181 l
h
f*
270.9354 511.3134 m
280.4598 511.3134 l
h
f*
248.1449 485.348 m
257.6693 485.348 l
h
f*
311.8677 472.3087 m
321.3921 472.3087 l
h
f*
323.6598 472.3087 m
333.1276 472.3087 l
h
f*
408.2457 446.3433 m
417.7134 446.3433 l
h
f*
419.9811 446.3433 m
429.5055 446.3433 l
h
f*
473.3858 407.3386 m
482.9102 407.3386 l
h
f*
203.4142 381.3165 m
212.9386 381.3165 l
h
f*
215.1496 381.3165 m
224.674 381.3165 l
h
f*
226.9417 381.3165 m
236.4661 381.3165 l
h
f*
391.9748 355.3512 m
401.4992 355.3512 l
h
f*
406.6583 355.3512 m
416.1827 355.3512 l
h
f*
482.0598 303.3071 m
491.5843 303.3071 l
h
f*
508.1953 277.3417 m
517.663 277.3417 l
h
f*
418.3937 264.3024 m
427.9181 264.3024 l
h
f*
362.3244 251.3197 m
371.7921 251.3197 l
h
f*
309.7134 225.2976 m
319.2378 225.2976 l
h
f*
362.8346 199.3323 m
372.3591 199.3323 l
h
f*
374.6268 199.3323 m
384.1512 199.3323 l
h
f*
463.5213 108.3402 m
472.989 108.3402 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(examine)Tj
3.6 0 Td
(the)Tj
1.4683 0 Td
(nanostructures)Tj
6.2249 0 Td
(of)Tj
1.0148 0 Td
(MA)Tj
1.7971 0 Td
(silk's)Tj
2.1657 0 Td
(proteins)Tj
3.5149 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(31)Tj
0 g
(].)Tj
3.5433 0 Td
(Of)Tj
1.2586 0 Td
(these)Tj
2.2507 0 Td
(only)Tj
1.9786 0 Td
(synchrotron-)Tj
-31.9009 -1.3039 Td
(derived)Tj
3.2144 0 Td
(SAXS)Tj
2.5058 0 Td
(and)Tj
1.7348 0 Td
(WAXS)Tj
2.9934 0 Td
(can)Tj
1.6271 0 Td
(explicitly)Tj
3.8324 0 Td
(and)Tj
1.7291 0 Td
(reliably)Tj
3.1691 0 Td
(measure)Tj
3.583 0 Td
(nanoscale)Tj
4.1442 0 Td
(variability)Tj
4.2236 0 Td
(in)Tj
1.0318 0 Td
(silk)Tj
1.5931 0 Td
(crys-)Tj
-35.3818 -1.2982 Td
(tallinity,)Tj
3.5035 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.474 0 Td
(size,)Tj
1.9105 0 Td
(density,)Tj
3.3222 0 Td
(orientation,)Tj
4.9152 0 Td
(and)Tj
1.7348 0 Td
(distance)Tj
3.5036 0 Td
(between)Tj
3.5263 0 Td
(individual)Tj
4.286 0 Td
(crystalline)Tj
4.2973 0 Td
(and)Tj
-34.2026 -1.2983 Td
(non-crystalline)Tj
6.2475 0 Td
(nanostructures)Tj
6.2248 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(33)Tj
0 g
(].)Tj
3.2485 0 Td
(For)Tj
1.6271 0 Td
(instance,)Tj
3.753 0 Td
(SAXS)Tj
2.5058 0 Td
(derived)Tj
3.2145 0 Td
(parameters)Tj
4.6771 0 Td
(such)Tj
2.058 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
-34.5711 -1.2983 Td
(meridional)Tj
4.6317 0 Td
(peak)Tj
2.092 0 Td
(and)Tj
1.7291 0 Td
(long)Tj
1.9842 0 Td
(period)Tj
2.8347 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
1.1508 0 Td
(used)Tj
2.058 0 Td
(to)Tj
1.0261 0 Td
(elucidate)Tj
3.7927 0 Td
(nanostructure)Tj
5.862 0 Td
(alignment)Tj
4.269 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(silk's)Tj
-35.5632 -1.3039 Td
(amorphous)Tj
4.7905 0 Td
(and)Tj
1.7291 0 Td
(lamellar)Tj
3.4412 0 Td
(regions)Tj
3.1635 0 Td
([)Tj
0.83 0.64 0.02 0 k
(34)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(36)Tj
0 g
(].)Tj
3.5489 0 Td
(WAXS)Tj
2.9991 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(other)Tj
2.3471 0 Td
(hand)Tj
2.2563 0 Td
(can)Tj
1.6271 0 Td
(be)Tj
1.1509 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(determine)Tj
-32.8816 -1.2982 Td
(silk)Tj
1.5873 0 Td
(crystallinity,)Tj
5.1194 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(size,)Tj
1.9106 0 Td
(density,)Tj
3.3278 0 Td
(and)Tj
1.7292 0 Td
(orientation,)Tj
4.9152 0 Td
(of)Tj
1.0148 0 Td
(crystalline)Tj
4.2973 0 Td
(nanostructures)Tj
6.2192 0 Td
(by)Tj
1.1848 0 Td
(exam-)Tj
-34.503 -1.2983 Td
(ining)Tj
2.313 0 Td
(the)Tj
1.474 0 Td
(scattering)Tj
4.1102 0 Td
(pattern)Tj
3.1124 0 Td
(and)Tj
1.7292 0 Td
(diffraction)Tj
4.439 0 Td
(angles)Tj
2.6986 0 Td
(\()Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(\))Tj
1.3663 0 Td
(at)Tj
0.9524 0 Td
(specific)Tj
3.1918 0 Td
(high)Tj
2.0069 0 Td
(intensity)Tj
3.685 0 Td
(diffraction)Tj
-31.0788 -1.3039 Td
(peaks)Tj
2.4434 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(38)Tj
0 g
(].)Tj
4.422 0 Td
(For)Tj
1.6158 0 Td
(spider)Tj
2.6815 0 Td
(silk)Tj
1.5817 0 Td
(the)Tj
1.4627 0 Td
(diffraction)Tj
4.4277 0 Td
(peaks)Tj
2.4434 0 Td
(at)Tj
0.9468 0 Td
(the)Tj
1.4627 0 Td
(\(200\),)Tj
2.5398 0 Td
(\(120\))Tj
2.3187 0 Td
(or)Tj
1.0942 0 Td
(\(002\))Tj
2.3187 0 Td
(regions)Tj
3.1521 0 Td
(identi-)Tj
-34.9112 -1.2983 Td
(fied)Tj
1.7234 0 Td
(on)Tj
1.2756 0 Td
(two)Tj
1.7121 0 Td
(dimensional)Tj
5.1704 0 Td
(WAXS)Tj
2.999 0 Td
(images)Tj
2.9877 0 Td
(are)Tj
1.4513 0 Td
(of)Tj
1.0148 0 Td
(particular)Tj
4.1102 0 Td
(interest)Tj
3.2088 0 Td
(as)Tj
1.0148 0 Td
(they)Tj
1.9276 0 Td
(are)Tj
1.4456 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
-34.2989 -1.2983 Td
(scattering)Tj
4.1102 0 Td
(from)Tj
2.2053 0 Td
(crystalline)Tj
/F12 1 Tf
4.2916 0 Td
()Tj
/F6 1 Tf
(sheets)Tj
3.6227 0 Td
([)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(39)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(41)Tj
0 g
(].)Tj
4.7225 0 Td
(SAXS)Tj
2.5058 0 Td
(and)Tj
1.7291 0 Td
(WAXS)Tj
2.999 0 Td
(accordingly)Tj
4.8869 0 Td
(are)Tj
1.4514 0 Td
(tools)Tj
2.1373 0 Td
(appro-)Tj
-34.6618 -1.3039 Td
(priate)Tj
2.5284 0 Td
(for)Tj
1.389 0 Td
(measuring)Tj
4.439 0 Td
(and)Tj
1.7292 0 Td
(classifying)Tj
4.3369 0 Td
(silks)Tj
1.9503 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(size,)Tj
1.9105 0 Td
(alignment,)Tj
4.4957 0 Td
(and)Tj
1.7348 0 Td
(distances,)Tj
4.0876 0 Td
(between)Tj
-33.8171 -1.2982 Td
(and)Tj
1.7291 0 Td
(within)Tj
2.8119 0 Td
(crystalline)Tj
4.2973 0 Td
(and)Tj
1.7291 0 Td
(non-crystalline)Tj
6.2476 0 Td
(nanostructures)Tj
6.2248 0 Td
([)Tj
0.83 0.64 0.02 0 k
(37)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(41)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(42)Tj
0 g
(].)Tj
-21.8436 -1.2983 Td
(X-ray)Tj
2.4831 0 Td
(scattering)Tj
4.1159 0 Td
(analyses)Tj
3.4809 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(MA)Tj
1.7972 0 Td
(silks)Tj
1.9502 0 Td
(of)Tj
1.0148 0 Td
(different)Tj
3.6567 0 Td
(spider)Tj
2.6872 0 Td
(species)Tj
2.999 0 Td
(has)Tj
1.5421 0 Td
(found)Tj
2.6192 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
-33.8057 -1.3039 Td
(micro-arrangement)Tj
8.0956 0 Td
(of)Tj
1.0148 0 Td
(silks)Tj
1.9503 0 Td
(from)Tj
2.2053 0 Td
(spiders)Tj
3.0501 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(Araneoidea)Tj
4.8415 0 Td
(clade)Tj
2.2734 0 Td
(are)Tj
1.4513 0 Td
(relatively)Tj
3.8381 0 Td
(conserved)Tj
-31.2262 -1.2983 Td
(across)Tj
2.6758 0 Td
(the)Tj
1.4684 0 Td
(group)Tj
2.6078 0 Td
([)Tj
0.83 0.64 0.02 0 k
(41)Tj
0 g
(].)Tj
2.0807 0 Td
(The)Tj
1.7801 0 Td
(mechanical)Tj
4.7678 0 Td
(properties)Tj
4.2803 0 Td
(of)Tj
1.0148 0 Td
(Araneoid)Tj
3.9798 0 Td
(spider)Tj
2.6873 0 Td
(MA)Tj
1.7971 0 Td
(silks,)Tj
2.177 0 Td
(nonetheless,)Tj
-31.3169 -1.2982 Td
(may)Tj
1.9275 0 Td
(vary)Tj
1.9332 0 Td
(considerably)Tj
5.2951 0 Td
(between)Tj
3.5263 0 Td
(species)Tj
2.999 0 Td
(and)Tj
1.7291 0 Td
(individuals)Tj
4.6488 0 Td
(across)Tj
2.6759 0 Td
(environments)Tj
5.794 0 Td
(and)Tj
1.7291 0 Td
(loading)Tj
3.2145 0 Td
(pre-)Tj
-35.4725 -1.2983 Td
(conditions)Tj
4.473 0 Td
([)Tj
0.83 0.64 0.02 0 k
(43)Tj
0 g
(].)Tj
2.0806 0 Td
(For)Tj
1.6214 0 Td
(instance,)Tj
3.7531 0 Td
(silk)Tj
1.5874 0 Td
(spun)Tj
2.1769 0 Td
(by)Tj
1.1793 0 Td
(spiders)Tj
3.05 0 Td
(walking)Tj
3.3619 0 Td
(along)Tj
2.4208 0 Td
(a)Tj
0.6576 0 Td
(surface)Tj
3.0444 0 Td
(is)Tj
0.8504 0 Td
(not)Tj
1.576 0 Td
(as)Tj
1.0148 0 Td
(stiff)Tj
1.7178 0 Td
(as)Tj
1.0148 0 Td
(that)Tj
-35.5802 -1.3039 Td
(spun)Tj
2.1713 0 Td
(by)Tj
1.1848 0 Td
(free)Tj
1.7235 0 Td
(falling)Tj
2.7156 0 Td
(spiders)Tj
3.05 0 Td
([)Tj
0.83 0.64 0.02 0 k
(43)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(44)Tj
0 g
(].)Tj
3.2542 0 Td
(Much)Tj
2.5908 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6725 0 Td
(variability)Tj
4.2236 0 Td
(is)Tj
0.8503 0 Td
(considered)Tj
4.5978 0 Td
(a)Tj
0.652 0 Td
(consequence)Tj
5.3404 0 Td
(of)Tj
-35.0416 -1.2983 Td
(changes)Tj
3.4015 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(friction)Tj
3.1918 0 Td
(acting)Tj
2.6532 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.474 0 Td
(silk)Tj
1.5874 0 Td
(as)Tj
1.0148 0 Td
(it)Tj
0.788 0 Td
(exits)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(valve)Tj
2.2337 0 Td
(during)Tj
2.9253 0 Td
(spinning)Tj
3.7474 0 Td
(since)Tj
2.2394 0 Td
(this)Tj
1.6781 0 Td
(induces)Tj
-34.2196 -1.2983 Td
(the)Tj
1.4683 0 Td
(amorphous)Tj
4.7905 0 Td
(region)Tj
2.8063 0 Td
(proteins)Tj
3.5149 0 Td
(to)Tj
1.0205 0 Td
(further)Tj
3.033 0 Td
(self-align)Tj
3.8495 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(43)Tj
0 g
(].)Tj
3.2541 0 Td
(The)Tj
1.7802 0 Td
(properties)Tj
4.2746 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(crystalline)Tj
-32.2807 -1.3039 Td
(nanostructures)Tj
6.2191 0 Td
(can)Tj
1.6271 0 Td
(nonetheless)Tj
4.8869 0 Td
(also)Tj
1.7688 0 Td
(vary)Tj
1.9389 0 Td
(within)Tj
2.8119 0 Td
(individual)Tj
4.286 0 Td
(spider)Tj
2.6872 0 Td
(silks)Tj
1.9503 0 Td
(across)Tj
2.6815 0 Td
(loading)Tj
3.2145 0 Td
(condi-)Tj
-34.0722 -1.2982 Td
(tions.)Tj
2.4264 0 Td
(For)Tj
1.6271 0 Td
(example,)Tj
3.7757 0 Td
(spiders)Tj
3.0444 0 Td
(exposed)Tj
3.4469 0 Td
(to)Tj
1.0261 0 Td
(winds)Tj
2.6079 0 Td
(of)Tj
1.0148 0 Td
(different)Tj
3.6566 0 Td
(strength)Tj
3.515 0 Td
(produce)Tj
3.5149 0 Td
(silks)Tj
1.9559 0 Td
(differing)Tj
3.6624 0 Td
(in)Tj
-35.2741 -1.2983 Td
(nanocrystal)Tj
4.8472 0 Td
(density,)Tj
3.3278 0 Td
(which)Tj
2.6476 0 Td
(affects)Tj
2.7382 0 Td
(the)Tj
1.4683 0 Td
(ultimate)Tj
3.532 0 Td
(strength)Tj
3.5149 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(fibers)Tj
2.4377 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
2.0807 0 Td
(Exposing)Tj
3.9174 0 Td
(spiders)Tj
3.0501 0 Td
(to)Tj
-36.0451 -1.3039 Td
(strong)Tj
2.7722 0 Td
(wind)Tj
2.245 0 Td
(induces)Tj
3.2995 0 Td
(silk)Tj
1.5931 0 Td
(extensibility)Tj
5.0456 0 Td
(and)Tj
1.7292 0 Td
(ultimate)Tj
3.5376 0 Td
(strength)Tj
3.5149 0 Td
(to)Tj
1.0205 0 Td
(change)Tj
3.0444 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(same)Tj
2.2564 0 Td
(direction)Tj
-32.5642 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(45)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(46)Tj
0 g
(].)Tj
4.4276 0 Td
(Simulations)Tj
4.972 0 Td
(and)Tj
1.7291 0 Td
(experiments)Tj
5.142 0 Td
(have)Tj
2.058 0 Td
(shown)Tj
2.8516 0 Td
(that)Tj
1.7745 0 Td
(variations)Tj
4.1669 0 Td
(in)Tj
1.0318 0 Td
(glandular)Tj
4.0251 0 Td
(pH,)Tj
1.7292 0 Td
(salts)Tj
1.9218 0 Td
(and)Tj
-35.8296 -1.2983 Td
(shear)Tj
2.3357 0 Td
(stress)Tj
2.3981 0 Td
(during)Tj
2.9253 0 Td
(spinning)Tj
3.7417 0 Td
(induce)Tj
2.9424 0 Td
(the)Tj
1.4683 0 Td
(poly-alanine)Tj
5.1987 0 Td
(residues)Tj
3.4809 0 Td
(to)Tj
1.0262 0 Td
(undergo)Tj
/F12 1 Tf
3.5886 0 Td
()Tj
/F6 1 Tf
(-helix)Tj
/F13 1 Tf
0 Tc
(!)Tj
/F12 1 Tf
-0.004 Tc
4.0705 0 Td
()Tj
/F6 1 Tf
(-sheet)Tj
-33.1764 -1.2982 Td
(phase)Tj
2.4831 0 Td
(transitions,)Tj
4.7055 0 Td
(which)Tj
2.6532 0 Td
(enhances)Tj
3.9061 0 Td
(silk)Tj
1.5931 0 Td
(strength)Tj
3.5149 0 Td
([)Tj
0.83 0.64 0.02 0 k
(47)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(50)Tj
0 g
(].)Tj
3.5433 0 Td
(These)Tj
2.5682 0 Td
(types)Tj
2.2733 0 Td
(of)Tj
1.0148 0 Td
(phase)Tj
2.4832 0 Td
(transitions)Tj
4.4843 0 Td
(could)Tj
-35.223 -1.3039 Td
(explain)Tj
3.118 0 Td
(the)Tj
1.474 0 Td
(enhancement)Tj
5.6353 0 Td
(of)Tj
1.0148 0 Td
(ultimate)Tj
3.5376 0 Td
(strength)Tj
3.5149 0 Td
(from)Tj
2.2054 0 Td
(spiders)Tj
3.05 0 Td
(exposed)Tj
3.4413 0 Td
(to)Tj
1.0261 0 Td
(high)Tj
2.0069 0 Td
(wind.)Tj
2.4775 0 Td
(Moreover,)Tj
4.3937 0 Td
(it)Tj
-36.8955 -1.2983 Td
(has)Tj
1.542 0 Td
(been)Tj
2.1203 0 Td
(shown)Tj
2.8516 0 Td
(that)Tj
1.7745 0 Td
(the)Tj
1.474 0 Td
(additional)Tj
4.2746 0 Td
(enhancement)Tj
5.6409 0 Td
(of)Tj
1.0148 0 Td
(extensibility)Tj
5.0457 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(silks)Tj
1.9502 0 Td
(of)Tj
1.0148 0 Td
(spiders)Tj
3.0501 0 Td
(exposed)Tj
-34.2593 -1.2983 Td
(to)Tj
1.0261 0 Td
(strong)Tj
2.7666 0 Td
(wind)Tj
2.2507 0 Td
(could)Tj
2.4491 0 Td
(be)Tj
1.1509 0 Td
(a)Tj
0.6576 0 Td
(consequence)Tj
5.3348 0 Td
(of)Tj
1.0148 0 Td
(spinning)Tj
3.7417 0 Td
(under)Tj
2.6192 0 Td
(a)Tj
0.6576 0 Td
(static)Tj
2.3017 0 Td
(load,)Tj
2.16 0 Td
(since)Tj
2.245 0 Td
(this)Tj
1.6725 0 Td
(induces)Tj
3.2995 0 Td
(the)Tj
-35.3478 -1.3039 Td
(amorphous)Tj
4.7905 0 Td
(region)Tj
2.8006 0 Td
(proteins)Tj
3.5149 0 Td
(to)Tj
1.0262 0 Td
(move)Tj
2.4264 0 Td
(more)Tj
2.3414 0 Td
(freely)Tj
2.4264 0 Td
(relative)Tj
3.1408 0 Td
(to)Tj
1.0261 0 Td
(each)Tj
2.024 0 Td
(other)Tj
2.3527 0 Td
([)Tj
0.83 0.64 0.02 0 k
(46)Tj
0 g
(].)Tj
-26.6738 -1.2982 Td
(An)Tj
1.457 0 Td
(additional)Tj
4.2746 0 Td
(experiment)Tj
4.7792 0 Td
(found)Tj
2.6192 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
1.4683 0 Td
(spider)Tj
/F11 1 Tf
2.6873 0 Td
[()28( )2()10(\n)18()4()4()]TJ
3.2768 0 Td
[()11()4()12()10()4( )8()]TJ
/F6 1 Tf
2.6645 0 Td
(produces)Tj
3.8778 0 Td
(stronger)Tj
3.566 0 Td
(and)Tj
-33.647 -1.2983 Td
(tougher)Tj
3.3391 0 Td
(MA)Tj
1.7972 0 Td
(silk)Tj
1.5931 0 Td
(when)Tj
2.4037 0 Td
(fed)Tj
1.457 0 Td
(a)Tj
0.652 0 Td
(high)Tj
2.0126 0 Td
(protein)Tj
3.1521 0 Td
(diet)Tj
1.7348 0 Td
(than)Tj
2.0296 0 Td
(when)Tj
2.4037 0 Td
(deprived)Tj
3.7304 0 Td
(of)Tj
1.0148 0 Td
(protein)Tj
3.1578 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
(Subsequent)Tj
-32.5528 -1.3039 Td
(WAXS)Tj
2.999 0 Td
(analyses)Tj
3.4809 0 Td
(found)Tj
2.6192 0 Td
(that,)Tj
2.0013 0 Td
(like)Tj
1.6554 0 Td
(wind)Tj
2.245 0 Td
(exposed)Tj
3.4469 0 Td
(spiders)Tj
3.0501 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(],)Tj
2.0749 0 Td
(changes)Tj
3.4072 0 Td
(in)Tj
1.0318 0 Td
(silk)Tj
1.5931 0 Td
(crystal)Tj
2.8063 0 Td
(density)Tj
-32.4111 -1.2983 Td
(explains)Tj
3.4809 0 Td
(some)Tj
2.33 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(enhanced)Tj
4.0762 0 Td
(strength)Tj
3.515 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
(Nevertheless,)Tj
5.4935 0 Td
(unlike)Tj
2.7326 0 Td
(wind)Tj
2.245 0 Td
(exposed)Tj
3.4469 0 Td
(spiders,)Tj
3.2712 0 Td
(a)Tj
-35.155 -1.2983 Td
(combination)Tj
5.3461 0 Td
(of)Tj
1.0148 0 Td
(changes)Tj
3.4015 0 Td
(in)Tj
1.0375 0 Td
(crystallinity)Tj
4.8925 0 Td
(and)Tj
1.7292 0 Td
(nanostructural)Tj
6.1171 0 Td
(orientation)Tj
4.6885 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(amorphous)Tj
-30.733 -1.3039 Td
(region)Tj
2.8006 0 Td
(were)Tj
2.1259 0 Td
(also)Tj
1.7632 0 Td
(prevalent)Tj
3.9401 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.075 0 Td
(In)Tj
1.1111 0 Td
(both)Tj
2.058 0 Td
(instances,)Tj
4.1102 0 Td
(the)Tj
1.4683 0 Td
(silk)Tj
1.5931 0 Td
(nanostructures)Tj
6.2248 0 Td
(seem)Tj
2.245 0 Td
(to)Tj
1.0262 0 Td
(vary)Tj
1.9332 0 Td
(inde-)Tj
-34.4747 -1.2982 Td
(pendent)Tj
3.5035 0 Td
(of)Tj
/F11 1 Tf
1.0148 0 Td
[()28()14()]TJ
/F6 1 Tf
(:)Tj
/F11 1 Tf
3.2032 0 Td
[()28()14()]TJ
/F6 1 Tf
2.9707 0 Td
(expression,)Tj
4.6941 0 Td
(although)Tj
3.7531 0 Td
(only)Tj
1.9729 0 Td
(indirect)Tj
3.3505 0 Td
(measures)Tj
3.9401 0 Td
(\(i.e.)Tj
1.7008 0 Td
(amino)Tj
2.7949 0 Td
(acid)Tj
1.8766 0 Td
(com-)Tj
-34.7752 -1.2983 Td
(position\))Tj
3.8324 0 Td
(of)Tj
/F11 1 Tf
1.0148 0 Td
[()28()14()]TJ
/F6 1 Tf
2.5058 0 Td
(expression)Tj
4.4673 0 Td
(was)Tj
1.7008 0 Td
(made)Tj
2.4208 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
3.2541 0 Td
(Different)Tj
3.8665 0 Td
(mechanisms)Tj
5.2044 0 Td
(at)Tj
0.9524 0 Td
(different)Tj
3.6567 0 Td
(scales)Tj
2.4718 0 Td
(seem)Tj
-35.3478 -1.2983 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1508 0 Td
(responsible)Tj
4.7509 0 Td
(for)Tj
1.3833 0 Td
(nutritionally)Tj
5.2384 0 Td
(induced)Tj
3.4639 0 Td
(spider)Tj
2.6872 0 Td
(silk)Tj
1.5931 0 Td
(property)Tj
3.6793 0 Td
(changes)Tj
3.4072 0 Td
(compared)Tj
4.2406 0 Td
(to)Tj
1.0262 0 Td
(wind)Tj
-33.647 -1.3039 Td
(induced)Tj
3.4639 0 Td
(property)Tj
3.6793 0 Td
(changes.)Tj
3.6283 0 Td
(However,)Tj
4.0819 0 Td
(no)Tj
1.2756 0 Td
(study)Tj
2.3867 0 Td
(has)Tj
1.5421 0 Td
(holistically)Tj
4.5014 0 Td
(examined)Tj
4.1272 0 Td
(the)Tj
1.4683 0 Td
(consequences)Tj
5.6976 0 Td
(of)Tj
-35.8523 -1.2983 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4617 0 Td
(on)Tj
1.2756 0 Td
(silk)Tj
1.593 0 Td
(proteins)Tj
3.515 0 Td
(and)Tj
1.7291 0 Td
(protein)Tj
3.1521 0 Td
(structure)Tj
3.8267 0 Td
(and,)Tj
1.9559 0 Td
(ultimately,)Tj
4.4561 0 Td
(silk)Tj
1.593 0 Td
(functional)Tj
4.3087 0 Td
(proper-)Tj
-33.9475 -1.2982 Td
(ties)Tj
1.5703 0 Td
(to)Tj
1.0262 0 Td
(understand)Tj
4.7848 0 Td
(the)Tj
1.4684 0 Td
(mechanisms)Tj
5.21 0 Td
(inducing)Tj
3.7927 0 Td
(nutritionally)Tj
5.2384 0 Td
(induced)Tj
3.4639 0 Td
(property)Tj
3.6794 0 Td
(variation)Tj
3.8041 0 Td
(in)Tj
1.0374 0 Td
(spider)Tj
-35.0756 -1.3039 Td
(silks)Tj
1.9502 0 Td
(at)Tj
0.9524 0 Td
(multiple)Tj
3.566 0 Td
(scales.)Tj
-5.2724 -1.2983 Td
(Here)Tj
2.211 0 Td
(we)Tj
1.3266 0 Td
(performed)Tj
4.4674 0 Td
(an)Tj
1.2018 0 Td
(examination)Tj
5.2328 0 Td
(of)Tj
1.0148 0 Td
(nutritionally)Tj
5.2383 0 Td
(induced)Tj
3.464 0 Td
(MA)Tj
1.7971 0 Td
(silk)Tj
1.5931 0 Td
(property)Tj
3.6793 0 Td
(variation)Tj
3.7984 0 Td
(by)Tj
-36.2208 -1.2983 Td
(running)Tj
3.4979 0 Td
(experiments)Tj
5.1363 0 Td
(similar)Tj
2.9877 0 Td
(to)Tj
1.0262 0 Td
(those)Tj
2.3357 0 Td
(described)Tj
4.0535 0 Td
(by)Tj
1.1792 0 Td
(Blamires)Tj
3.7247 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
(However,)Tj
4.0762 0 Td
(we)Tj
1.3266 0 Td
(used)Tj
2.0637 0 Td
(five)Tj
-35.5519 -1.3039 Td
(species)Tj
2.9933 0 Td
(of)Tj
1.0148 0 Td
(Araneoid)Tj
3.9855 0 Td
(spiders:)Tj
/F11 1 Tf
3.2768 0 Td
[()30()6()-8()4()11()4( )]TJ
3.2428 0 Td
[()8( )-5()2()-3( )2()8()12()12()13()-8()]TJ
/F6 1 Tf
(,)Tj
/F11 1 Tf
4.5298 0 Td
[()16()-9()4()11()10(\n)6()11()20()]TJ
4.1612 0 Td
[()7()20()16()10()6()16()-9()12()6()]TJ
/F6 1 Tf
(,)Tj
/F11 1 Tf
5.3518 0 Td
[()-3()22()7()20()4()4( )7(\r)-4()7()-4()]TJ
4.7735 0 Td
[(\n)6()10()-3( )15()14()7()]TJ
/F6 1 Tf
(,)Tj
/F11 1 Tf
-33.3295 -1.2982 Td
[()28( )2()10(\n)18()4()4()]TJ
3.2768 0 Td
[()10()12()12()18()10()4( )8()]TJ
/F6 1 Tf
3.702 0 Td
(and)Tj
/F11 1 Tf
1.7291 0 Td
[()4(\n)6()11()6()2()-8()6()22()10(\n)6()]TJ
5.4539 0 Td
[()-8()20()6( )1()9( )2()]TJ
/F6 1 Tf
(,)Tj
3.2881 0 Td
(and)Tj
1.7348 0 Td
(directly)Tj
3.2315 0 Td
(measured)Tj
4.1045 0 Td
(spidroin)Tj
3.5887 0 Td
(expression)Tj
4.4673 0 Td
(using)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 388.5732 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Multiscale)Tj
4.585 0 Td
(mechanism)Tj
5.0316 0 Td
(s)Tj
0.7157 0 Td
(of)Tj
1.0418 0 Td
(spider)Tj
2.8913 0 Td
(silk)Tj
1.63 0 Td
(property)Tj
3.8268 0 Td
(variatio)Tj
3.1535 0 Td
(n)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01920)Tj
4.089 0 Td
(05)Tj
0 g
2.0906 0 Td
(February)Tj
4.1599 0 Td
(1,)Tj
1.0417 0 Td
(2018)Tj
33.7042 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
105 0 obj
<>stream
endstream
endobj
106 0 obj
<>stream
endstream
endobj
107 0 obj
<>stream
endstream
endobj
108 0 obj
<>stream
endstream
endobj
109 0 obj
<>stream
endstream
endobj
110 0 obj
<>stream
endstream
endobj
111 0 obj
<>stream
endstream
endobj
112 0 obj
<>stream
endstream
endobj
113 0 obj
<>stream
endstream
endobj
114 0 obj
<>stream
endstream
endobj
115 0 obj
<>stream
endstream
endobj
116 0 obj
<>stream
endstream
endobj
117 0 obj
<>stream
endstream
endobj
118 0 obj
<>stream
endstream
endobj
119 0 obj
<>stream
endstream
endobj
120 0 obj
<>stream
endstream
endobj
121 0 obj
<>stream
endstream
endobj
122 0 obj
<>stream
endstream
endobj
123 0 obj
<>stream
endstream
endobj
124 0 obj
<>stream
BT
66.402 0 Td
(23)Tj
ET
Q
endstream
endobj
125 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 12 0 R/Annots 131 0 R/Contents 132 0 R/TrimBox[0 0 612 792]>>
endobj
130 0 obj
<>/ProcSet[/PDF/ImageC]/Properties<>/XObject<>>>/Filter/FlateDecode/Length 148>>stream
hޔ=0ݧx;mҬ1t@CZ
lI,}G;l
6]K . vR f=9Y: QpE W"1 ÕŰS>%KRX뗥[kꏥok۷e> A2
endstream
endobj
133 0 obj
<>
endobj
134 0 obj
<<>>
endobj
135 0 obj
<>stream
h Ul I %(/8tw&CYRzݝtn?*
a!d4KGtU5YzSu&{^4uT|;'xzo_@ o_7Pq~xE
w#bz]=={{~@89F?O(((>ӻ}ٿ??rd~L?mUx7?L{ov}|0oǞxqrfq3ޠo,RCc؈MOnh4"
s̐|^GAAAAAAgDb0k)S6p_ $O&h})&
wO{465Tˠ8
.}
?51o
\ѷ{7>a
Jl6xh٦8C6ظq/.Ic$dI:AAAQ#~Zo$L ۻgO>9I)(D-FAAAAA7\<~!6Őq=)Sx{]T5^6LvT993{ xe`Ts$LC̆zٴ_|Y5g%b,]GD>0y]Mb3ΕjcΓΗc'Ŝ`T|AAAywml8.zk~9-Alݻh8hd$b|y6rU̚<<{ՁlAAwZg"s/̙9Ό/X{XGO%u[3kkNήmkVyʝϖW'h' ⸳T~z5DWg̨cCeL߽a`?|9 A46&q՜)Ou /JWsmW$WNGDAqB]>3OjF
-ۆٷѓzkX=鰛.Zvu[lwm!"e{Ǟ5
SmURͅ0`K] 0s\r.@n 8\uM^,7-|TsЅ$!oٸɃLՓy~ݎM,~f bP3#z`s"bBd۫&PR(J' 9[
lye00
8hV=\ڭum!"e{O_?xmxT8o*0ׁ8.fd JS* ̜1V9mh6
up }SOjme~s3-Al :bbC3nժI<B Ne/J[3gjmd{ 3
v-Kkc1ɧ.|%\
qq$#|{<6h3P,Tv&AAcʕ_,N͜;^?0@@qCٷfBD6AAll6AA$qBv tm./*VF
;{ ?yW]I=7>y^zCk][